Deletion

PAM Type:

Accept the range for protospacers search(it is necessary when there is no suitable PAM for destination editing)

Region for nick of pegL from the desired start position (the default values are recommended):

Region for nick of pegR from the desired end position (the default values are recommended):

Prime binding site (PBS) length: 7-30

RT template length (the default values are recommended):

Recommended Tm of PBS sequence (℃):

Tm-directed PBS length model

Exclude first C in RT template

Note: Deletions are indicated by "(a/)", where "a" is removed.The ellipsis '...' is optional, used for brevity to indicate omitted portions of the sequence. When using ellipsis, the lengths between the ellipsis and the parentheses must exceed the values set by the 'Accept the range for spacers search' sub-options.

FASTA File:

Primer Design:

TaU3 OsU3 CmYLCV Other

Forward primer (5'-3'):

+ Spacer sequence +

Reverse primer (5'-3'):

+ PBS and RT template +